ISS超分辨激光共焦扫描显微镜Alba-STED

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测试实例展示Alba-STEDforFLIM/FFS

Confocal(left)vs.pSTED(right)imagesoftheactinlabeledwiththeSiRdyeinfixedgliacells,acquiredbyFastFLIM.

Confocal(left)vs.pSTED(right)imagesoftheactinlabeledwiththeSiRdyeinfixedgliacells,acquiredbyFastFLIM.

Confocalimagesof60nmfluorescencebeads(left);pSTEDimages(middle);sharpeningthepSTEDimageusingabinaryfilterbasedonthephasorplots(right).

DuallabelscanbeseparatedusingpSTEDandFastFLIM.Atto647NandAtto655wereusedaslabels;theybothareexcitedbythe640nmlaser.Thetwodyesarefirstseparatedusingthephasorplots,andthenassignedwithtwodifferentfalsecolors(Atto647N-yellow,Atto655-purple)toproducetheprocessedandmergedpSTEDimageofthetwolabels.

参考论文：

Monomericcohesinstaterevealedbylive-cellsingle-moleculespectroscopy.

Liu,W.,Biton,E.,Pathania,A.,Matityahu,A.,Irudayaraj,J.,Onn,I.

EMBORep.2019Dec29:e48211.doi:10.15252/embr.201948211.[Epubaheadofprint]

Photon-separationtoenhancethespatialresolutionofpulsedSTEDmicroscopy.

Tortarolo,G.,Sun,Y.,Teng,K.W.,Ishitsuka,Y.,Lanzanó,L.,Selvin,P.R.,Barbieri,B.,Diaspro,A.,Vicidomini,G.

Nanoscale.2019Jan9.doi:10.1039/c8nr07485b.[Epubaheadofprint]

AstraightforwardSTED-backgroundcorrectedfittingmodelforunbiasedSTED-FCSanalyses.

Wang,R.,Brustlein,S.,Mailfert,S.,Fabre,R.,Fallet,M.,Sivankutty,S.,Rigneault,H.,Marguet,D.

Methods.2018May1;140-141:212-222.doi:10.1016/j.ymeth.2018.02.010.Epub2018Feb14.

ANovelPulsedSTEDMicroscopyMethodUsingFastFLIMandthePhasorPlots

Sun,Y.,TortaroloG.,Teng,K.-W.,Ishitsuka,Y.,Coskun,U.C.,Liao,S.-C.,Diaspro,A.,Vicidomini,G.,Selvin,P.R.,Barbieri,B.

Proc.SPIE10069,MultiphotonMicroscopyintheBiomedicalSciencesXVII,100691C(February21,2017)