See All Kinase Enzyme Systems 
Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TOPK is a MAPK kinase that phosphorylates p38 MAPK  is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro (1). Expression of TOPK is detected in male germ line progenitor cells, activated T-cells  a variety of lymphomas  leukemias. In vitro studies have shown that activated TOPK phosphorylates p38 MAPK but not JNK  ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex  another unknown kinase, possibly Raf C  Raf A. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation  differentiation (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted  ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount  kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase  ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: TOPK, 10μg (Human, recombinant full-length). MW: ~68kDa.

• Substrate: Native Swine Myelin Basic Protein (MBP).

• Other: Reaction Buffer, DTT.

TOPK NCBI Database Entry.

Human Kinase Enzyme Systems  Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up  running in no time.

• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT  supplemental reagents as needed.

• Obtain Reliable Results: The broad dynamic range, the ease of use  better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.