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详细说明
产品类型:
ELISA Kit
产品名称:
小鼠核因子E2相关因子2(Nrf2)ELISA Kit
英文名称:
Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit
货号:
CSB-E16188m
别名:
NRF2, NF-E2-related factor 2|nuclear factor erythroid 2-like 2
规格:
96T / 48T
种属:
Mouse
待测物名称: nuclear factor (erythroid-derived 2)-like 2
缩写:
NFE2L2
蛋白功能1:
Transcription/Transcription regulation
蛋白功能3:
Transcription
检测范围:
0.312 ng/ml-20 ng/ml
灵敏度:
0.078 ng/ml
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml
OD1
OD2
Average
Corrected
20
2.648
2.880
2.764
2.627
10
1.966
1.916
1.941
1.804
5
1.357
1.291
1.324
1.187
2.5
0.963
0.945
0.954
0.817
1.25
0.514
0.547
0.531
0.394
0.625
0.358
0.345
0.352
0.215
0.312
0.233
0.239
0.236
0.099
0
0.138
0.135
0.137
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. 线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Nrf2 in various matrices diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 83-94 1:2 Average % 102 Range % 96-107 1:4 Average % 99 Range % 93-104 1:8 Average % 92 Range % 86-99 回收率:
The recovery of mouse Nrf2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 82-95 EDTA plasma (n=4) 95 90-102 研究领域:
Cardiovascular
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