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详细说明
概述
产品名称Anti-C20orf7抗体[EPR15870] - C-terminal
描述
兔单克隆抗体[EPR15870] to C20orf7 - C-terminal
经测试应用IHC-P,ICC/IF,IP,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human C20orf7 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q5TEU4Run BLAST with
Run BLAST with 
阳性对照
HepG2, 293, HeLa, Jurkat, Human skeletal muscle and fetal liver, mouse spleen, brain, heart, kidney, NIH 3T3 and Raw264.7 cell lysates; Human endometrium and Human breast carcinoma tissues; NIH 3T3 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15870
同种型IgG
Anti-C20orf7 antibody [EPR15870] - C-terminal 图像
![Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192235235.jpg)
Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)
All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 38 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.![Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535b.jpg)
Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)
All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 38 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.![Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535c.jpg)
Western blot - Anti-C20orf7 antibody [EPR15870] (ab192235)
All lanes : Anti-C20orf7 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : RAW 264.7 lysate
Lane 6 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 38 kDa
Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535d.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C20orf7 antibody [EPR15870] (ab192235)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling C20orf7 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535e.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C20orf7 antibody [EPR15870] (ab192235)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling C20orf7 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
![Immunocytochemistry/ Immunofluorescence - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535f.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C20orf7 antibody [EPR15870] (ab192235)
Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling C20orf7 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-C20orf7 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.![Immunoprecipitation - Anti-C20orf7 antibody [EPR15870] (ab192235)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19223535g.jpg)
Immunoprecipitation - Anti-C20orf7 antibody [EPR15870] (ab192235)
Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.
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