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详细说明
概述
产品名称Anti-HSPA4抗体[EPR14165]
描述
兔单克隆抗体[EPR14165] to HSPA4
经测试应用Flow Cyt,IP,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 100-200. The exact sequence is proprietary.
Database link: P34932阳性对照
K562 cells and cell lysates; HepG2 cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14165
同种型IgG
研究领域
Metabolism
Pathways and Processes
Redox metabolism
Oxidative stress
Cell Biology
Other Antibodies
Oxidative Stress
Signal Transduction
Protein Trafficking
Chaperones
Heat Shock Proteins
Anti-HSPA4 antibody [EPR14165] 图像
![Western blot - Anti-HSPA4 [EPR14165] antibody (ab185219)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185219219.JPG)
Western blot - Anti-HSPA4 [EPR14165] antibody (ab185219)
All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 94 kDa
![Flow Cytometry - Anti-HSPA4 [EPR14165] antibody (ab185219)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185219192.JPG)
Flow Cytometry - Anti-HSPA4 [EPR14165] antibody (ab185219)
Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-HSPA4 [EPR14165] antibody (ab185219)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185219193.JPG)
Immunoprecipitation - Anti-HSPA4 [EPR14165] antibody (ab185219)
Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
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