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详细说明
概述
产品名称Anti-HSPA4抗体[EPR14166]
描述
兔单克隆抗体[EPR14166] to HSPA4
经测试应用IHC-P,ICC/IF,WB,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPA4 aa 700-800. The exact sequence is proprietary.
Database link: P34932阳性对照
HeLa and HepG2 cells and cell lysates; K562, Human cerebellum, Mouse heart, Rat brain and Rat heart lysates; Human brain tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14166
同种型IgG
研究领域
Metabolism
Pathways and Processes
Redox metabolism
Oxidative stress
Cell Biology
Other Antibodies
Oxidative Stress
Signal Transduction
Protein Trafficking
Chaperones
Heat Shock Proteins
Anti-HSPA4 antibody [EPR14166] 图像
![Western blot - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962962.JPG)
Western blot - Anti-HSPA4 [EPR14166] antibody (ab185962)
All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 94 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962624.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 [EPR14166] antibody (ab185962)
Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
![Western blot - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962opy.jpg)
Western blot - Anti-HSPA4 [EPR14166] antibody (ab185962)
All lanes : Anti-HSPA4 antibody [EPR14166] (ab185962) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 94 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962623.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 [EPR14166] antibody (ab185962)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling HSPA4 with ab185962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962625.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 [EPR14166] antibody (ab185962)
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
![Flow Cytometry - Anti-HSPA4 [EPR14166] antibody (ab185962)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185962626.JPG)
Flow Cytometry - Anti-HSPA4 [EPR14166] antibody (ab185962)
Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling HSPA4 with ab185962 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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