• Species Reactivity

  Human

• Specificity

  Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

• Source

  Monoclonal Mouse IgG    ₁ Clone # B4-78

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human peripheral blood granulocytes

• 
  

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human intestine

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

• References:

1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.

2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.

3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

• Long Name:

  Alkaline Phosphatase Liver

• Entrez Gene IDs:

  249 (Human); 11647 (Mouse)

• Alternate Names:

  Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935