• Species Reactivity

  Human

• Specificity

  Detects human Cathepsin V in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Cathepsin B, C, F, L, O, S, or Z is observed. This antibody specifically identifies the pro form but not the active form of Cathepsin V in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 182021

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Cathepsin V    
  Val18-Val334    
  Accession # O60911

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Cathepsin V (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Cathepsin V (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin V

Cathepsin V, also known as cathepsin L2 and cathepsin U, is a lysosomal cysteine protease expressed in the thymus, testis, and corneal epithelium. It may play a role in tumor progression since it is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues. Additionally, it may be involved in corneal physiology. The amino acid sequence of human Cathepsin V shares 99%, 83% and 76% identical with that of chimpanzee, dog and mouse/rat, respectively.

• Entrez Gene IDs:

  1515 (Human)

• Alternate Names:

  Cathepsin L2; Cathepsin U; Cathepsin V; CATL2; CTSL2; CTSUcathepsin L2, preproprotein; CTSVMGC125957; EC 3.4.22; EC 3.4.22.43